Cytotoxic and Anti-proliferative Effects of Ethanol Extract of Marine Sponge Stylissa carteri on Colon Cancer HCT-116 Cell Line

Colorectal cancer is one of the most diagnosed cancers in world. KRAS mutations colon are being responsible for progressiveness and resistance standard therapeutic available. Marine sponge sources chemotherapy. Stylissa carteri a marine that lives Indonesia its anti-cancer effects starting to be explored nowadays. The purpose this study was determine cytotoxicity anti-proliferative effect ethanol extract against cells with HCT-116 cells. This an vitro using cell line which tested by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) trypan blue assay. assay performed on control treated at 72 h. MTT were previously incubated extract. Sponge taken from Pramuka island, Taman Nasional Kepulauan Seribu, Jakarta. IC50 5 μg/mL (R square 0.9550) showed doubling time 48 h 26.10 39.69 cells, respectively. Ethanol has cytotoxic mutation.Keywords: Colon Cancer, HCT-116, Mutation, Sponge, carteri.